DNA Sequencing Protocols

DNA Sequencing Protocols

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DNA sequencing; M13 cloning vehicles: their contribution to DNA sequencing; Cloning into M13; Transfection of E.coli with M13DNA.Col 7: T7 DNA polymerase (5 U/uL), 6 I¼L/cycle. 6. Col 8: ddA termination ... Troubleshooting for T7 Sequencing In general, each series of sequencing ... In the following troubleshooting guide it is assumed that the control reaction works. 3.3.1.

Title:DNA Sequencing Protocols
Author: Hugh G. Griffin
Publisher:Springer Science & Business Media - 1993

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