DNA Cloning: Expression systems

DNA Cloning: Expression systems

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Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modernmolecular biology laboratories.bacteriophage CE6, a lambda phage recombinant containing and expressing the T7 polymerase gene. ... 2.4 pCE vectors This series of vectors may be used as alternative histidine tagged vectors to allow affinity purification of fusion proteins on Niaquot; resin columns. ... rearrangements of plasmid DNA have been reported in DH5 derivative or XL-1 blue strains (Pharmacia GST troubleshooting guide). The tacanbsp;...

Title:DNA Cloning: Expression systems
Author: David M. Glover, B. D. Hames
Publisher:Oxford University Press - 1995

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